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Widal Test (SERUM)

Widal test is done to diagnose the enteric fever (Typhoid fever). Enteric fever is a life-threatening illness caused by infection with the bacterium Salmonella enterica serotype Typhi (S. typhi), usually transmitted through food and drinks contaminated with fecal matter. It is associated with symptoms that include high fever, fatigue, headache, abdominal pain, diarrhea or constipation, weight loss, and a rash known as "rose spots." Early diagnosis and treatment are important because serious complications, including severe intestinal bleeding or perforation, can develop within a few weeks.Widal test is developed in 1896 and named after Georges Ferdinand Widal, who introduced it, the method relies on a reaction in a test tube or on a slide between antibodies present in the infected person's blood sample and specific antigens of S. typhi, which produces clumping (agglutination) that is visible to the naked eye. While the method is easy to perform, concerns remain about the reliability of the Widal test and studies of the assay's sensitivity and other measures of reliability have been disappointing. Besides cross-reactivity with other Salmonella species, the test cannot distinguish between a current infection and a previous infection or vaccination against typhoid.

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Common Names

Typhoid test; Salmonella Antibodies Blood Test; Typhoid Fever Antibodies Blood Test.

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Disease Conditions

Typhoid; Enteric Fever

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Why To Test

The Widal test is a blood test, widely used for serological diagnosis of enteric fever (typhoid / paratyphoid fever). It measures the titre (level) of antibodies (in blood of patients) against salmonella antigens:

  • TO ? Somatic (O) antigen of Salmonella typhi
  • TH ? Flagellar (H) antigen of Salmonella typhi
  • AH ? Flagellar (H) antigen of Salmonella Paratyphi
  • A BH Flagellar (H) antigen of Salmonella Paratyphi B
    The antibodies against O antigen of only Salmonella typhi are measured, since all Salmonella have similar O antigens. An increasing O antibody level signifies acute infection, whilst a raised H antibody level may indicate the serotype of the infecting organism, or an earlier infection. However, as for any other serological test, it has many limitations as a diagnostic tool, since it provides indirect evidence (as a surrogate marker) for infection. Also, the rise in antibody levels, needed to make the diagnosis, takes 7-14 days, which limits its use. The interpretation of the serology thus requires consideration of factors such as previous immunisation, the stage of the illness and the effect of any antibiotic treatment, infection with any other gram-negative bacteria, etc. As a diagnostic marker, Widal test must be done on paired serum samples. The first sample is taken early in the disease and the second sample at least 2 weeks later. A four-fold rise in antibodies in the paired samples is significant and diagnoses acute recent infections. Widal test can also be used in carrying out serological surveys in a community to know the prevalence (and endemicity in an area) of enteric fever and determine the cut off titre for interpretation in that population. The traditional Widal test is done in tubes (tube agglutination test) and needs 15-18 hours for the test to be done. A modified Widal test is commercially available as a slide agglutination test. However, the isolation of Salmonella bacteria in the blood (by blood culture) is the gold standard for diagnosing enteric fever.
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When To Test

Following are the clinical indications for performing the Widal Blood Test:

  • Fever, chills
  • Fatigue
  • Headache
  • Abdominal pain
  • Diarrhea
  • Constipation
  • Weight loss
  • Rash spots 2-4 mm in diameter, called ?rose spots?
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About Us

iHealthMantra

We at iHealthMantra enable patients to book their diagnostics tests from best Diagnostics Labs in India such as Thyrocare, Dr Lal Pathlabs, Metropolis, Suburban Diagnostics, Medall Clumax and many others top quality diagnostic labs. Diagnostics tests booked through us are at low price and patients can avail free home sample collection.

We also provide preventive health checkups, Regular Health check-up, Health checkup packages, Thyroid Profile, PET Scan, MRI Scan, CT Scan, Full body check up, Vitamin D Profile, Lipid Profile, Renal Function Tests and other blood tests. Provided below is a brief description of few of our partner labs.

About Thyrocare

Thyrocare, with its tag line of Think Thyroid, Think Thyrocare, is India's first fully automated diagnostic laboratory with a focus on providing quality at affordable costs to retail customers, laboratories and hospitals in India and other countries. Thyrocare has strong focus on technology, brand and systems that enables Thyrocare laboratories to give their clients the best of science and technology at an affordable cost. Thyrocare laboratories have NABL and CAP accreditations. Thyrocare supports home sample collection to provide convenience to customers.Thyrocare is a PAN India chain with services across all the major Indian cities such New Delhi, Mumbai, Pune, Bangalore, Chennai, Hyderabad and Kolkata.

Key tests and health profiles supported by Thyrocare are: T3, T4, TSH also called Thyroid Profile, TSH also called Thyroid Stimulating Hormone, Thyrocare Aarogyam Packages such as Mini Aarogyam, Aarogyam A, Aarogyam B, Aarogyam C, Vitamin Profiles such as Vitamin D Profile and Vitamin D3 test.

About Metropolis

Metropolis is India?s one of the biggest and multinational chain of pathology labs and diagnostics centre known for excellent customer satisfaction and preventive healthcare. Metropolis has following accreditations, CAP, NABL and RNTCP. Metropolis Healthcare has been honoured for its best practices and its commitment to excellence in customer satisfaction and innovation. The journey of Metropolis started in 1981, when its Founder and Chairman, Dr. Sushil Shah had a vision to introduce specialized testing facilities to the Indian population. Processing over 30 million tests a year, catering to over 20,000 Laboratories, Hospitals, Nursing Homes and 2,00,000 consultants; and with 34 years of experience in delivering accurate reports, Metropolis has earned the reputation of being India's multinational chain of laboratories with a strong presence in Sri Lanka, UAE, South Africa, Kenya, Mauritius & Ghana. Metropolis supports home sample collection to provide convenience to customers. Metropolis is a PAN India chain with services across all the major Indian cities such New Delhi, Mumbai, Pune, Bangalore, Chennai and Hyderabad.

Common Tests supported by Metropolis are Lipid Profile, Thyroid Profile, Vitamin D Profile, Renal function test, Blood Tests, Vitamin D3, TSH, T3, T4, Diabetes Test, ECG test, thalassemia test, preventive health checkups, Regular Health check-up, Health checkup packages and Full body check up.

About Dr Lal Pathlabs

Dr Lal PathLabs is a leading Diagnostic Centre and Pathology Lab for Blood Test in India. Dr Lal PathLabs has a strong focus on quality with NABL accreditation. Dr Lal PathLabs has a qualified team of Pathologists, Radiologists, Microbiologists, Biochemists and specialists. Dr Lal PathLabs supports home sample collection to provide convenience to customers. Dr Lal PathLabs is a PAN India chain with services across all the major Indian cities such New Delhi, Mumbai, Pune, Bangalore, Chennai, Hyderabad and Kolkata.

Common Tests supported by Dr Lal PathLabs are Lipid Profile, Thyroid Profile, Vitamin D Profile, Renal function test, Blood Tests, Vitamin D3, TSH, T3, T4, Diabetes Test, ECG test, thalassemia test, preventive health checkups, Regular Health check-up, Health checkup packages and Full body check up. Few unique health packages supported by Dr Lal PathLabs are Swasth Plus Basic Health Checkup, Swasth Plus Advanced Health Checkup and Swasth Plus Vitamins Package.

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Customer Support

We can help and guide you to find answer to your queries. Some sample questions we answered for our customers are: What is Widal Test (SERUM) cost in Mumbai? Cost of Widal Test (SERUM) in Mumbai? What is Widal Test (SERUM) cost in Bangalore? Cost of Widal Test (SERUM) in Bangalore ? What is Widal Test (SERUM) cost in Pune? Cost of Widal Test (SERUM) in Pune? What is Widal Test (SERUM) cost in Hyderabad? Cost of Widal Test (SERUM) in Hyderabad? What is Widal Test (SERUM) cost in Delhi? Cost of Widal Test (SERUM) in Delhi? What is Widal Test (SERUM) cost in Ahmedabad? Cost of Widal Test (SERUM) in Ahmedabad? You can also call us at 9503472446 or email us at customercare@ihealthmantra.com or chat with our customer support.

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