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HIV Screening Tests; AIDS Test; AIDS Screen; HIV Serology; p24 Antigen; HIV-1 and HIV-2 Antibody and Antigen Evaluation.

HIV Screening Tests; AIDS Test; AIDS Screen; HIV Serology; p24 Antigen; HIV-1 and HIV-2 Antibody and Antigen Evaluation.


Human immunodeficiency virus (HIV) is the cause of AIDS (acquired immunodeficiency syndrome). HIV screening tests detect the HIV antigen (p24) and/or HIV antibodies produced in response to an HIV infection in the blood. Some tests detect HIV antibody in oral fluid.

When a person becomes infected with HIV, through exposure to the blood or body fluids of an infected individual or a contaminated needle, for example, the virus begins to replicate itself, producing a large number of copies. During the first few weeks of infection, the amount of virus (viral load) and the p24 antigen level in the blood can be quite high.

About 2-8 weeks after exposure to the virus, the immune system responds by producing antibodies directed against the virus that can be detected in the blood. As the initial infection resolves and the level of HIV antibody increases, both virus and p24 antigen levels decrease in the blood.

An HIV infection may initially cause no symptoms or cause flu-like symptoms that resolve after a week or two. The only way to determine whether a person has been infected is through HIV testing.

If HIV is not detected early and treated, it may become a simmering infection that may cause few symptoms for a decade or more. If the infection is still not treated, eventually symptoms of AIDS emerge and begin to progressively worsen. Over time and without treatment, HIV destroys the immune system and leaves a person's body vulnerable to debilitating infections. (You can read more about this in the article on HIV Infection and AIDS.)

Detecting and diagnosing HIV early in the course of infection is important because:

It allows for early treatment that slows progression to AIDS. An individual can learn of their status and modify behavior so as to prevent the spread of disease. A pregnant woman can undergo treatment that would help prevent passing the disease to her child. There are two types of HIV, 1 and 2. HIV-1 is the most common type found in the United States, while HIV-2 has a higher prevalence in parts of Africa.

A few different testing options are available for HIV screening:

Combination HIV antibody and HIV antigen test?the recommended screening test for HIV; it is available only as a blood test. HIV antibody testing?all HIV antibody tests used in the U.S. detect HIV-1 and some tests have been developed that can also detect HIV-2. These tests are available as blood tests or tests of oral fluid. p24 antigen testing?this is used alone without the antibody test only in rare cases when there is a question about interference with an HIV antibody test. Regardless of the type of screening test used, a positive result requires follow up with supplemental testing to establish a diagnosis of HIV. (For more details, see "The Test" section.)

Report delivery time

20 minutes


Testing for p24 allows for detection of early infections, before HIV antibody is produced. A few weeks after exposure, antibodies to HIV are produced in response to the infection and remain detectable in the blood thereafter, making the antibody test useful for detecting infections weeks after exposure.

Common uses

HIV; AIDS; Sexually Transmitted Disease

Customer support

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